Biotechnology Research Group

• Prof. Dr. Ülkü MEHMETOĞLU
• Prof. Dr. Emine BAYRAKTAR
• Prof. Dr. Afife GÜVENÇ

PhD Students
• Res. Assist. Rahime SONGÜR

Ms Students
• Pınar AK
• Günay BAYDAR

Ø Fermentation Process
-Organic Acid, Amino Acid, Antibiotic Production
-Extractive Fermentation,
Ø Mathematical Modeling of Bioreactors
Ø Enzymatic and Microbial Biotransformation
-Amino Acid and Aroma Compound Production,
-Production of Enantiomerically Pure Pharmaceuticals
-Production of Flavor and Fragrance Esters
-Extractive Biotransformation
Ø Bioseparation Methods
-Reactive Extraction,
-Reverse Micelle Systems
-Gas Hydrate Methods
Ø Application of Supercritical CO 2 Technology to Biochemical Systems and Reactions
-Extraction of Fermentation Products
-Extraction of Natural Products
-Enzymatic Production of Flavor and Fragrance Esters
Ø Application of Ultrasound on Biochemical Reactions
Ø Biochemical Production using Plant Cell Culture
Ø Cleaning of Waste
-Using Biochemical Methods,
– Using Supercritical CO2


1. Improvement of Enantioselective a -Hydroxy Ketone Production Process by Biocatalitacally (Çiğdem Babaarslan)

1. Kapucu, H. and Mehmetoğlu, Ü., 1999. “The effects of Bioprocess Parameters on the Yield in Extractive Ethanol Fermentation”, Reviews in Chemical Engineering, 15, 4, 307-318.
2. Bayraktar E., Çiçek B.and Mehmetoğlu Ü., 1999. “Mathematical Modelling of a Packed Bed Bioreactor for the Citric Acid Production”, Reviews in Chemical Engineering, 15 ,4, 279-292.
3 . Güvenç A., Mehmetoğlu Ü., Çalımlı A.,. 1999. “Supercritical CO 2 Extraction of Ethanol”, Turkish Journal of Chemistry, 23(3), p.285-291.
4. Kapucu,N,. Güvenç A., Mehmetoğlu Ü., and Çalımlı A., 1999. “Reactive Extraction of Organic Acids by Supercritical CO 2 ”, Reviews in Chemical Engineering, 15, 4, 233-243.
5. Bayraktar,E., Mehmetoğlu Ü., 2000. “Production of Citric Acid Using Immobilized Conidia of Aspergillus niger ”, Applied Biochemistry and Biotechonology, 87 , 2, 117-125.
6. Kapucu, H., Gülsoy, N.,and Mehmetoğlu, Ü., 2000. Disruption and protein release kinetics by ultrasonication of Acetobacter peroxydans cells, Biochemical Engineering Journal,5, 1, 57-62.
7. Bayraktar,E., 2001.“Response Surface Optimization of the Separation of DL- Typtophan Using an Emulsion Liquid Membrane”, Process Biochemistry, 37, 169-175.
8. Bayraktar, E., Mehmetoğlu Ü., 2001 .“Back Mixing and Liquid Hold-up Up-flow Packed Bed Bioreactor”, Chemical Engineering Communications, 185 , 125-140.
9. Kahya,E., Bayraktar E. and Mehmetoğlu Ü., 2001. “Optimization of Process Parameters for the Reactive Lactic Acid Extraction”, Turkish Journal of Chemistry, 25 , 223-230.
10. Tik, N., E. Bayraktar and Ü. Mehmetoğlu, 2001 . “In-situ Reactive Extraction of Lactic Acid from Fermentation Media” J.Chem. Technol. Biotechnol., 76, 764-768.
11. A. Koparan, A. Güvenç, N. Kapucu, Ü., Mehmetoğlu, ve A. Çalımlı, 2001, “Separation process of citric acid with tertiary amines / diluents in supercritical CO 2 ” , Turkish Journal of Chemistry , 25(4), p.477-484.
12. Kılıç,M., Bayraktar E., Ateş S., Mehmetoglu Ü., 2002. “Investigation of Extractive Citric Acid Fermentation Using Response-surface Methodology”,Process Biochemistry, 37 ,759-767.
13. Güvenç, A., Kapucu, N. and Mehmetoğlu, Ü., 2002. “The Production of Isoamyl Acetate Using Immobilized Lipases in Solvent-free System”, Process Biochemistry, 38,379-386.
14. Ateş,S., Dingil, N., Bayraktar, E., Mehmetoglu, Ü. 2002., “Enhancement of Citric Acid Production using Silicone Oil by Immobilized and Freely Suspended Aspergillus niger” , Process Biochemistry, 38, 433-436.
15. Salgın U, Üstün A.S., Mehmetoğlu Ü. and Çalımlı A., 2002. “Supercritical Fluid Extraction of Secondary Plant Metabolites from Capsicum annuum L.”, Technology of Health Care (International Journal of Health Care Engineering), 10 (3,4), 357-359
16. Guvenç,A., Kapucu, N., Bayraktar,E. and Mehmetoğlu,Ü. 2003. “Optimization of The Enzymatic Production of Isoamyl Acetate with Novozym 435 from Candida antarctica”, Chemical Engineering Communications, 190 , 5-8, 948-961.
17. Kapucu,N., Guvenç,A., Kapucu,H., Mehmetoğlu,Ü. and Çalımlı,A., 2003. “Lipase Catalyzed Synthesis of Oleyl Oleate : Optimization by Response Surface Methodology” , Chemical Engineering Communications, 190, 779-796.
18. AbuHamed,T., Bayraktar,E. Mehmetoğlu,T. and Mehmetoğlu,Ü., 2003. “Substrate Interactions During The Biodegradation of Benzene, Toluene And Phenol Mixtures” Process Biochemistry, 39, 27-35.
19. Çelik,D., Bayraktar,E., and Mehmetoğlu,Ü., 2003.“Biotransformation Of 2-Phenylethanol To Phenylacetaldehyde In A Two-Phase Fed-Batch System”, Biochemical Engineering Journal, 17, 5-13
20. Döker,O. Bayraktar,E., Mehmetoğlu,Ü. and Çalmlı,A., 2003. “Production of Iron-Cobalt Compound Nanoparticles using Reverse Micellar System”, Rev. Adv. Mater. Sci. 5, 34-40.
21. Babaarslan, Ç., Abuhamed, T., Tekeli, A., Mehmetoğlu, T., Mehmetoğlu, Ü.,2003, “Biodegradation of BTEX Compounds by a Mixed Culture Obtained from Petroleum Formation Water”, Energy Sources, 25, 7, 733-742.
22. Döker, O., Salgın, U., Şanal, İ., Mehmetoğlu, Ü. and Çalımlı, A., 2004. Modeling of extraction of ß-carotene from apricot bagasse using supercritical CO 2 in packed bed extractor, The Journal of Supercritical Fluid, 28, 111-119.
23. A., Güvenç, Ü.,Mehmetoğlu, T., Mehmetoğlu, 2004. “Effects of Operational Parameters and Ultrasonic Pretreatment on Supercritical CO 2 Extraction of Diesel Fuel From Soil”, Energy Sources , 26(1), 77-82.
24. Abuhamed, T, Bayraktar, E, Mehmetoğlu, T. and Mehmetoğlu, Ü, 2004 . “The Biodegradation of Benzene, Toluene and Phenol in a Two-phase System”, Biochemical Engineering Journal, 19, 137-146.
25. Abuhamed, T, Bayraktar, E, Mehmetoğlu, T. and Mehmetoğlu, Ü, 2004.“ Kinetics Model for Growth of Pseudomonas putida F1 During Benzene, Toluene and Phenol Biodegradation”, Process Biochemistry ,39, 983-988.
26. İ.S. Şanal, A. Güvenç, U. Salgın, Ü. Mehmetoğlu, A. Çalımlı, 2004. “Recycling of Apricot Pomace by Supercritical CO 2 Extraction”, The Journal of Supercritical Fluids (in press).

PROJECTS (going on)
1. Enantioselective production of a -hydroxy Ketones using Enzymes and Microorganisms (Ankara University Biotechnology Institution Project, 36, 2002).
2. Production of nano sized ferro compounds. Ankara University Scientific Research Projects (2003.07.45.021), 2004-
3. Kinetic resolution of 1-phenyl 1-propanol by enantioselective esterification with enzyme and microbial cells ” Ankara University Biotechnology Project, 2005-

-Gas Chromatography (CE Instruments, CA 8000 Top)
-Liquid Chromatography, HPLC, (Thermo Finnigan, spectra)
-UV Spectrophotometer (Shimadzu, 1601 A)
-Refrigerated Centrifuge (Hettich, Rotina 35 R)
-Sterile Cabin (CE Heto-Holten )
-Orbital Shaker (Gerhardt)
-Refrigerated Incubator (Shel Lab)
-Incubator (Nüve)
-Microscope (Olympus CHK-2)
-Ultra Sonicator (Cole Parmer)
-Refrigerated Circulator (Nüve, BS 302)
-Rotary Evaporator (Heidolph-labor, OTA 4000)
-Automatic Titrator (Orion)
– Sterilizator
-Deep freeze (SANYO MDF-U5186S)
-Bioreactor (BIOSTAT CT)
-Water activity equipment (AW SPRINT, Novasina)
-Batch supercritical extraction /reaction system
-Peristaltic pumps


• Biotechnology Research Laboratory
• Research Laboratory
• New Technologies Laboratory